Indian Council of Agricultural Research (ICAR) on Tuesday announced that it has developed India’s first fully annotated telomere-to-telomere (T2T) reference genome of pigeonpea (Cajanus cajan L. Millsp.) variety ‘Asha’.
The newly developed T2T genome sequence comprises 752.65 million base pairs, assembled into 92 contigs, providing a complete representation of all 11 chromosomes of pigeonpea. The genome assembly, designated NIPB_CcT2T_4, has been deposited in the global database (NCBI), ICAR said in a statement.
The genome assembly comprises all 11 chromosomes, including their centromeres and telomeres, demonstrating chromosome-level completeness. A comprehensive genome annotation identified 36,557 genes, producing 48,008 mRNAs (coding sequences). Transcriptome (RNA sequence) mapping recorded more than 99.9 per cent read alignment, confirming the high accuracy and completeness of the genome.
